| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 2641 | Thermobifida fusca | AAGCCGACCTCAAAGCGT | CTGGACAGCATGCCGATCT | 59.57 | 59.85 | 187 | 34 |
100.00%
|
100.00%
|
| Primer 2642 | Thermobifida fusca | GCGACTTCATGATGAGCGC | ACGACGAGATGTGCACGT | 59.72 | 59.36 | 228 | 34 |
100.00%
|
100.00%
|
| Primer 2643 | Thermobifida fusca | GGCCTGTCCTGCTGATGTT | AATCCCTGCCAGCGGATT | 60.00 | 58.99 | 287 | 34 |
100.00%
|
100.00%
|
| Primer 2644 | Thermobifida fusca | CCGTCACGGGAAAACTCGT | TCCGAAAGAGCAGTTGGCC | 60.30 | 60.30 | 161 | 34 |
100.00%
|
100.00%
|
| Primer 2645 | Thermobifida fusca | GCACCTTGGACGTGGACAT | CAATACCAGGTCGTCGCGT | 60.30 | 60.15 | 215 | 34 |
100.00%
|
100.00%
|
| Primer 2646 | Thermobifida fusca | ATGCCCACTGTTTCCGCT | ACGCTCATCCGCATTCGT | 59.56 | 59.82 | 284 | 34 |
100.00%
|
100.00%
|
| Primer 2647 | Thermobifida fusca | ATGCCCACTGTTTCCGCT | ACCACGCTCATCCGCATT | 59.56 | 59.73 | 287 | 34 |
100.00%
|
100.00%
|
| Primer 2648 | Thermobifida fusca | TGCAGTTGGTTCCTCACCC | AATTCACCGACGACGCCT | 59.85 | 59.35 | 277 | 33 |
100.00%
|
100.00%
|
| Primer 2649 | Thermobifida fusca | TTTCACCGACCAGCCGTT | ACCAGCGCAGTTCGTAGT | 59.49 | 58.95 | 164 | 33 |
100.00%
|
100.00%
|
| Primer 2650 | Thermobifida fusca | TCCTCGACGAGTGTGACCT | GCAGAAACCAGGCGTGAAC | 59.93 | 59.72 | 239 | 33 |
100.00%
|
100.00%
|